Bacteriophages are viruses that affect bacteria and not plants, humans,s or any other mammals; due to their specificity, it poses no harm to other creatures except that specific bacteria host. To determine whether which laboratory can handle which bacteriophage depends on the host used instead of the phage managed, therefore the determinant is bacteria and not the virus. For example, bacteriophages for Mycobacterium must be handled in higher labs since the host (the deadly bacteria) will be used at one point. Let’s polish our knowledge on bio-safety laboratory levels.
Bio-safety Level 1
Biosafety level one, the lowest level, applies to work with agents that usually pose a minimal potential threat to laboratory workers and the environment and do not consistently cause disease in healthy adults. Research with these agents is generally performed on standard open laboratory benches without special containment equipment. SL 1 labs are not usually isolated from the general building. Training on the specific procedures is given to the lab personnel, supervised by a trained microbiologist or scientist.
Standard microbiology practices are usually enough to protect laboratory workers and other employees in the building. These include mechanical pipetting only (no mouth pipetting allowed), safe sharps handling, avoidance of splashes or aerosols, and decontamination of all work surfaces when work is complete, e.g., daily. Decontamination of spills is done immediately, and all potentially infectious materials are decontaminated before disposal, generally by autoclaving. Standard microbiological practices also require attention to personal hygiene, i.e., hand washing and a prohibition on eating, drinking, or smoking in the lab. Standard laboratory personal protective equipment is generally worn, including eye protection, gloves, and a lab coat or gown. Biohazard signs are posted, and access to the lab is limited whenever infectious agents are present.
Bio-safety Level 2
Bio-safety Level 3
Bio-safety Level 4
|Biosafety lab levels by CDC