|Phage display. source Wikipedia|
Phage display is a molecular biology technique that uses in vitro screening to find protein and other macromolecule ligands. A gene encoding a protein of interest is inserted into a phage coat protein gene, causing the phage to “display” the protein on the outside while containing the protein’s gene on the inside, resulting in a link between genotype and phenotype. This technique has evolved over many years with significant advancement to become one of the most important techniques in protein engineering.
In this technique, in vitro selection can be accomplished by screening and amplifying large protein libraries, which is analogous to natural selection. Phage display is also a powerful method for producing large quantities of peptides, proteins, and antibodies. E.coli filamentous bacteriophages (f1, fd, M13) are the most commonly used in phage display, though T4, T7, and phage have also been used.
G. Smith invented this technique in 1985 as a means of presenting polypeptides on the surface of lysogenic filamentous bacteriophages.
Applications of Phage Display
Epitope mapping and mimicking
Identification of new receptors & ligands
Protein-protein interaction studies
Recombinant Antibody Production
a. Production of gene fragment
b. Cloning of gene fragments in the phagemid vectors
c. Selection of specific phages
Protein directed evolution
In vitro diagnostic
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