PREPARATION

Preparation of LB (Luria-Bertani) agar

Media, PREPARATION, PROTOCOL

Luria-Bertani is the most preferred media to be used in phage research. This protocol describes how...
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Bacterial Stock Culture preparation

Cultures, PREPARATION, PROTOCOL

Procedure Prior to the procedure, a stock culture of the phage target bacteria should be maintained on any general media preferably...
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Preparation of anti-phage agent

PREPARATION, PROTOCOL, Reagents

  Procedure Immediately prior to use, transfer 3.3 ml of 13% PRE added to 7 ml of freshly prepared ferrous sulphate solution (0.01%)....
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Preparation of 13% Pomegranate Rind Extract (PRE)

PREPARATION, PROTOCOL, Reagents

 Procedure Blend pomegranate rind in distilled water (25% w/v) and boil for 10 min. Centrifuge...
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Preparation of FeSO4.7H2O Solution

PREPARATION, PROTOCOL, Reagents

Procedure First freshly prepare a solution (0.01%) of FeSO4.7H2O in Lambda buffer, pH 6.3. Sterilized by membrane filtration (0.45 lm,...
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Preparation of SM buffer (lambda diluent)

Buffers, PREPARATION, PROTOCOL

SM buffer is primarily used in biology laboratories, and its main function, like any other buffer, is to stabilize the pH. That is, it can...
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Preparation of Phosphate Buffered Saline (PBS)

Buffers, PREPARATION, PROTOCOL

Phosphate-buffered saline (abbreviated PBS) is a buffer solution commonly used in biological...
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How is phage cocktail prepared?

AMR, PHAGE RESISTANCE, PHAGE TECHNOLOGY, PREPARATION

One of the significant advantages of bacteriophages as antimicrobials is their specificity. A consequence of this characteristic is that...
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How to make an outstanding media (agar) plate

PREPARATION

Whether they contain TSA, MHA, LB, M9, or any other medium, making agar plates is a simple procedure. But there are a few finer points that...
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preparation of Gelatin solution, 2%

PREPARATION

Gelatin solution, 2% To prepare a 2% (w/v) solution: Add 2 g of gelatin to a total volume of 100 ml...
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