Techniques to evaluate bacteriophage killing
Bacteriophages are viruses that are particularly reliant on their bacterial hosts’ metabolic...
The best way to Isolate bacteriophage DNA
As someone who has done a lot of bacteriophage research, I know how common it is to get host DNA contamination when attempting to isolate...
Isolation of Pseudomonas bacteriophages from fish gastro intestinal tract
Fish plays a vital role in the human diet, and consumption per capita is increasing; however, the expansion of industry and agriculture...
Isolation of edwardsiella-specific bacteriophages from fish
Aquaculture, the culture of all types of aquatic animals and plants in fresh, back rush, and marine...
Agar Overlay (double layer agar) technique
Preparation of an overlay This method creates a homogeneous lawn of bacteria within a thin layer of agar across the surface of a plate....
Preparation of LB (Luria-Bertani) agar
Luria-Bertani is the most preferred media to be used in phage research. This protocol describes how to make 1 L of Luria-Bertani agar (also...
Plaque-Based Optimization of the Phages Lytic Characteristics
The isolates of wild lytic phages from the transient stocks are propagated with the corresponding...
Production of the Transient Phage Stock
Procedure Cut 5–10 plaques with a sterile Pasteur pipette and place them in 1.5 ml sterile Eppendorf micro-centrifuge tubes. Add...
Phage Spot Lysis Test
Procedure Prepare bacterial lawns of target bacterial isolates or reference bacterial strains by adding 500 µl of LB 18 h cultures...
Procedure for Phage isolation
Procedure Collect crude specimens of approximately 50 g or 100mls of any targeted sample in...
