PROTOCOL

Techniques to evaluate bacteriophage killing

PROTOCOL

Bacteriophages are viruses that are particularly reliant on their bacterial hosts’ metabolic...
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The best way to Isolate bacteriophage DNA

PROTOCOL • 2 Comments

As someone who has done a lot of bacteriophage research, I know how common it is to get host DNA contamination when attempting to isolate...
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Isolation of Pseudomonas bacteriophages from fish gastro intestinal tract

PROTOCOL, STUDENT'S PERSPECTIVE • 2 Comments

Fish plays a vital role in the human diet, and consumption per capita is increasing; however, the expansion of industry and agriculture...
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Isolation of edwardsiella-specific bacteriophages from fish

PROTOCOL, STUDENT'S PERSPECTIVE • One Comment

Aquaculture, the culture of all types of aquatic animals and plants in fresh, back rush, and marine...
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Agar Overlay (double layer agar) technique

PROTOCOL

Preparation of an overlay This method creates a homogeneous lawn of bacteria within a thin layer of agar across the surface of a plate....
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Preparation of LB (Luria-Bertani) agar

Media, PREPARATION, PROTOCOL

Luria-Bertani is the most preferred media to be used in phage research. This protocol describes how to make 1 L of Luria-Bertani agar (also...
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Plaque-Based Optimization of the Phages Lytic Characteristics

PROTOCOL, Techniques

The isolates of wild lytic phages from the transient stocks are propagated with the corresponding...
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Production of the Transient Phage Stock

PROTOCOL, Techniques

Procedure  Cut 5–10 plaques with a sterile Pasteur pipette and place them in 1.5 ml sterile Eppendorf micro-centrifuge tubes. Add...
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Phage Spot Lysis Test

PROTOCOL, Techniques

Procedure  Prepare bacterial lawns of target bacterial isolates or reference bacterial strains by adding 500 µl of LB 18 h cultures...
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Procedure for Phage isolation

PROTOCOL, Techniques • One Comment

  Procedure Collect crude specimens of approximately 50 g or 100mls of any targeted sample in...
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