Plaque-Based Optimization of the Phages Lytic Characteristics
The isolates of wild lytic phages from the transient stocks are propagated with the corresponding...
Production of the Transient Phage Stock
Procedure Cut 5–10 plaques with a sterile Pasteur pipette and place them in 1.5 ml sterile Eppendorf micro-centrifuge tubes. Add...
Phage Spot Lysis Test
Procedure Prepare bacterial lawns of target bacterial isolates or reference bacterial strains by adding 500 µl of LB 18 h cultures...
Procedure for Phage isolation
Procedure Collect crude specimens of approximately 50 g or 100mls of any targeted sample in...
