Techniques

Plaque-Based Optimization of the Phages Lytic Characteristics

PROTOCOL, Techniques

The isolates of wild lytic phages from the transient stocks are propagated with the corresponding...
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Production of the Transient Phage Stock

PROTOCOL, Techniques

Procedure  Cut 5–10 plaques with a sterile Pasteur pipette and place them in 1.5 ml sterile Eppendorf micro-centrifuge tubes. Add...
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Phage Spot Lysis Test

PROTOCOL, Techniques

Procedure  Prepare bacterial lawns of target bacterial isolates or reference bacterial strains by adding 500 µl of LB 18 h cultures...
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Procedure for Phage isolation

PROTOCOL, Techniques • One Comment

  Procedure Collect crude specimens of approximately 50 g or 100mls of any targeted sample in...
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