Bioluminescence-based Reporter Bacteriophages: New Technology for Monitoring Microbes and Diseases March 26, 2022 Bacteria and bacteriophage. Photo by Oxford (Article by Pete Wilton) In recent years, the development of bioluminescence-based reporter bacteriophages has revolutionized the monitoring of microbes…
The potential use of Bacteriophage Lysins In the Medical field March 20, 2022 Structure of gram-negative bacteriophage lysin. credit Fischetti (2019) Phage lysins are specialized hydrolytic cell wall enzymes that are used to rupture phage-infected bacteria and release…
Are Phages Microbiome-Friendly Therapeutic Approach? March 4, 2022 Introduction Antibiotics are the most frequently prescribed class of drugs in the US and many other parts of the world. However, they have become…
Can the Bacteriophage Mutants be the Next Generation Carriers for Conjugate Vaccines? February 26, 2022 Passing through several waves of the deadly pandemic brought the human race into consideration of doing extensive research on vaccines. It is no doubt…
How do bacteriophages transfer antibiotic resistance genes (ARGs)? February 24, 2022 An illustration of ARG transfer by T bacteriophages Because of the rise in antibiotic resistance, effective prevention of pathogens is becoming a pipe dream….
The accessibility of phage therapy services January 27, 2022 where do people get phage therapy? Despite the reinstated attention it has gained in recent years, Phage therapy is still limited to only some…
DendriPep: Innovative macromolecules that stabilize bacteriophages as a medicine January 16, 2022 In the world of booming antimicrobial resistance, scientists worldwide struggle to find a proper method of treating superbugs. Recently, the savior has been recognized…
Preparation of LB (Luria-Bertani) agar January 1, 2022 Luria-Bertani is the most preferred media to be used in phage research. This protocol describes how to make 1 L of Luria-Bertani agar (also…
Plaque-Based Optimization of the Phages Lytic Characteristics December 26, 2021 The isolates of wild lytic phages from the transient stocks are propagated with the corresponding host bacterial isolates and the representative standard reference bacterial…
Production of the Transient Phage Stock December 26, 2021 Procedure Cut 5–10 plaques with a sterile Pasteur pipette and place them in 1.5 ml sterile Eppendorf micro-centrifuge tubes. Add into 300 µl of…