How do bacteriophages transfer antibiotic resistance genes (ARGs)? February 24, 2022 An illustration of ARG transfer by T bacteriophages Because of the rise in antibiotic resistance, effective prevention of pathogens is becoming a pipe dream….
The accessibility of phage therapy services January 27, 2022 where do people get phage therapy? Despite the reinstated attention it has gained in recent years, Phage therapy is still limited to only some…
DendriPep: Innovative macromolecules that stabilize bacteriophages as a medicine January 16, 2022 In the world of booming antimicrobial resistance, scientists worldwide struggle to find a proper method of treating superbugs. Recently, the savior has been recognized…
Preparation of LB (Luria-Bertani) agar January 1, 2022 Luria-Bertani is the most preferred media to be used in phage research. This protocol describes how to make 1 L of Luria-Bertani agar (also…
Plaque-Based Optimization of the Phages Lytic Characteristics December 26, 2021 The isolates of wild lytic phages from the transient stocks are propagated with the corresponding host bacterial isolates and the representative standard reference bacterial…
Production of the Transient Phage Stock December 26, 2021 Procedure Cut 5–10 plaques with a sterile Pasteur pipette and place them in 1.5 ml sterile Eppendorf micro-centrifuge tubes. Add into 300 µl of…
Procedure for Phage isolation December 26, 2021 Procedure Collect crude specimens of approximately 50 g or 100mls of any targeted sample in a sterile sample collection tube (100 ml). Mix…
Bacterial Stock Culture preparation December 26, 2021 Procedure Prior to the procedure, a stock culture of the phage target bacteria should be maintained on any general media preferably Trypticasein soy agar(TSA),…
Preparation of anti-phage agent December 26, 2021 Procedure Immediately prior to use, transfer 3.3 ml of 13% PRE added to 7 ml of freshly prepared ferrous sulphate solution (0.01%). After…
Preparation of 13% Pomegranate Rind Extract (PRE) December 26, 2021 Procedure Blend pomegranate rind in distilled water (25% w/v) and boil for 10 min. Centrifuge (20,000xg, 4 °C, 30 min) (A further purification of…
