Preparation of FeSO4.7H2O Solution December 26, 2021 Procedure First freshly prepare a solution (0.01%) of FeSO4.7H2O in Lambda buffer, pH 6.3. Sterilized by membrane filtration (0.45 lm, Whatman)
Preparation of SM buffer (lambda diluent) December 26, 2021 SM buffer is primarily used in biology laboratories, and its main function, like any other buffer, is to stabilize the pH. That is, it…
Preparation of Phosphate Buffered Saline (PBS) December 26, 2021 Phosphate-buffered saline (abbreviated PBS) is a buffer solution commonly used in biological research including phage work. It is a water-based salt solution containing disodium…
Common media used in phage work December 26, 2021 microbiological Media List of the media used Luria broth (LB): tryptone 10 g l−1 (HiMedia, Mumbai, India), yeast extract5 g l−1 (HiMedia, Mumbai, India),…
The structure, packaging and ejection of bacteriophage genome December 24, 2021 Bacteriophages consist of a protein coat that surrounds/protects their genomes; the arrangement of the genome inside this coat has been proposed by several researchers….
Can lysogenic phages be trained to enter into the lytic cycle? October 10, 2021 Have you ever come across the word phage training? Phages are viruses that eat up bacteria; that’s what many of us know. They are…
The adventure of bacteriophage adsorption to the bacteria and the brownian motion October 2, 2021 How do phages adsorb to bacteria? Did you know that phage adsorption to bacterial cells involves Brownian motion? If no, then you are just…
Review: Can phages have a third life cycle? September 23, 2021 Have you ever heard of bacteriophages (phages)? If yes, then what about Pseudolysogeny? The two words are in one way or the other interconnected…
Antiviral properties of bacteriophages July 24, 2021 Bacteriophages are viruses known to attack bacteria. Their effectiveness in eradicating the most antibiotic-resistant bacteria piqued scientists’ interest in the prospect of using them…
Which kind of lab is supposed to handle bacteriophages? June 17, 2021 Bacteriophages are viruses that affect bacteria and not plants, humans,s or any other mammals; due to their specificity, it poses no harm to other…
