Production of the Transient Phage Stock December 26, 2021 Procedure Cut 5–10 plaques with a sterile Pasteur pipette and place them in 1.5 ml sterile Eppendorf micro-centrifuge tubes. Add into 300 µl of…
Procedure for Phage isolation December 26, 2021 Procedure Collect crude specimens of approximately 50 g or 100mls of any targeted sample in a sterile sample collection tube (100 ml). Mix…
Bacterial Stock Culture preparation December 26, 2021 Procedure Prior to the procedure, a stock culture of the phage target bacteria should be maintained on any general media preferably Trypticasein soy agar(TSA),…
Preparation of anti-phage agent December 26, 2021 Procedure Immediately prior to use, transfer 3.3 ml of 13% PRE added to 7 ml of freshly prepared ferrous sulphate solution (0.01%). After…
Preparation of 13% Pomegranate Rind Extract (PRE) December 26, 2021 Procedure Blend pomegranate rind in distilled water (25% w/v) and boil for 10 min. Centrifuge (20,000xg, 4 °C, 30 min) (A further purification of…
Preparation of FeSO4.7H2O Solution December 26, 2021 Procedure First freshly prepare a solution (0.01%) of FeSO4.7H2O in Lambda buffer, pH 6.3. Sterilized by membrane filtration (0.45 lm, Whatman)
Preparation of SM buffer (lambda diluent) December 26, 2021 SM buffer is primarily used in biology laboratories, and its main function, like any other buffer, is to stabilize the pH. That is, it…
Preparation of Phosphate Buffered Saline (PBS) December 26, 2021 Phosphate-buffered saline (abbreviated PBS) is a buffer solution commonly used in biological research including phage work. It is a water-based salt solution containing disodium…
Common media used in phage work December 26, 2021 microbiological Media List of the media used Luria broth (LB): tryptone 10 g l−1 (HiMedia, Mumbai, India), yeast extract5 g l−1 (HiMedia, Mumbai, India),…
The structure, packaging and ejection of bacteriophage genome December 24, 2021 Bacteriophages consist of a protein coat that surrounds/protects their genomes; the arrangement of the genome inside this coat has been proposed by several researchers….
