The multiplicity of infection (MOI) of the phage explained April 6, 2021 The Multiplicity of Infection (MOI) is a crucial metric in bacteriophage research and is defined as the ratio of the number of infectious agents…
Preparation of Phage Lysates / bacteriophage enumeration (pfu/ml) March 24, 2021 Bacteriophage enumeration is a crucial laboratory technique used to quantify the abundance of bacteriophages, viruses that infect and replicate within bacteria. This process typically…
Isolation of Phage DNA – Miniprep Protocol March 24, 2021 Extracting the DNA from a bacteriophage is essential in order to study its genetic makeup and identify the genes that encode its various…
Determination of phage adsorption time March 14, 2021 The determination of phage adsorption time is a critical step in the study of phages and their interactions with bacteria. This information can provide…
preparation of Gelatin solution, 2% March 8, 2021 Gelatin solution, 2% To prepare a 2% (w/v) solution: Add 2 g of gelatin to a total volume of 100 ml of H2O and…
Preparation of Tris-Cl March 8, 2021 Tris base HCl To prepare a 1 M solution, dissolve 121.1 g of Tris base in 800 mL of H2O. Adjust the pH…
Preparation of SM Buffer without gelatin March 8, 2021 SM buffer is primarily used in biology laboratories and, like any other buffer, its primary function is to stabilize the pH. It can withstand…
Comprehensive Guide to Bacteriophage Counting through Plaque Assay February 25, 2021 Principle of Phage Plaque Assay When a suspension of an infective phage (e.g. T4 phage) is spread over the lawn of susceptible bacterial cells…
