There is a recent study about the extraction and detection of phage DNA from a sputum sample done by scientists from Stanford University (Bacteriophages can transmit antibiotic resistance genes through the multiplication process). This study proves that bacteriophages are real everywhere and are numerous compared to their natural prey (bacteria). There is increasing interest in the pulmonary microbiome’s bacterial and viral communities, particularly in the context of chronic airway infections in cystic fibrosis (CF). However, the isolation of microbial DNA from the sputum from patients with CF is technically challenging and the optimal protocols for the analysis of viral species, including bacteriophage, from clinical samples, remains difficult.
In the study, they evaluated a set of methods developed for processing and analyzing sputum from patients with CF with the goal of detecting Pf bacteriophage virion-derived nucleic acid. they also evaluated the impact of bead beating, deoxyribonuclease digestion, and heating steps in these protocols focusing on the quantitative assessment of Pseudomonas aeruginosa (Pseudomonas spp are one of the most antibiotic-resistant bacteria to be recorded in the past five years) and Pf bacteriophage in sputum.
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